Language Theories free essay sample

Examines ideas of Ferdinand de Saussure, Jacques Lacan Sigmund Freud related to linguistic, psychological semiotic interpretations of the individual culture. The purpose of this research is to examine the theories of Ferdinand de Saussure, Jacques Lacan, and Sigmund Freud as they relate to linguistic, psychological, and semiotic interpretations of the individual and of the culture as a whole. The plan of the research will be to set forth a summary of Saussures theory of semiotics and the outlines of Freudian psychological theory, and then to discuss the connection between the work of Lacan and Freud in regard to analysis of human subjectivity, as well as the connection between Lacans work to linguistic theory in general and Saussurian semiotics in particular. According to Saussure, language has a dual function. One is public, or a logical and social, while the other is private, imaginative, or psychological. It is in the second manner that creative and imaginative processes may surface, including the

Biography of Francisco Madero, Led Mexican Revolution

Biography of Francisco Madero, Led Mexican Revolution Francisco I. Madero (October 30, 1873–February 22, 1913) was a reformist politician and writer and president of Mexico from 1911 to 1913. This unlikely revolutionary helped engineer the overthrow of dictator Porfirio Dà ­az by kick-starting the Mexican Revolution. Unfortunately for Madero, he was caught between remnants of Dà ­azs regime and the revolutionaries he unleashed and was deposed and executed in 1913. Fast Facts: Francisco Madero Known For: Father of the Mexican RevolutionBorn: Oct. 30, 1873 in Parras, MexicoParents: Francisco Ignacio Madero Hernndez, Mercedes Gonzlez Trevià ±oDied: Died Feb. 22, 1913 in Mexico City, MexicoSpouse: Sara Pà ©rez Early Life Francisco I. Madero was born on Oct. 30, 1873, in Parras, Coahuila, Mexico, to wealthy parents- by some accounts, the fifth-richest family in Mexico. His father was Francisco Ignacio Madero Hernndez; his mother was Mercedes Gonzlez Trevià ±o. His grandfather, Evaristo Madero, made lucrative investments and was involved in ranching, wine-making, silver, textiles, and cotton. Francisco was well educated, studying in the United States, Austria, and France. When he returned from the U.S., he was placed in charge of some family interests, including the San Pedro de las Colonias hacienda and farm, which he operated at a profit, introducing modern farming methods and improving worker conditions. In January 1903, he married Sara Pà ©rez; they had no children. Early Political Career When Bernardo Reyes, governor of Nuevo Leà ³n, brutally broke up a political demonstration in 1903, Madero became politically involved. Although his early campaigns for office failed, he funded a newspaper that he used to promote his ideas. Madero had to overcome his image to succeed as a politician in macho Mexico. He was small with a high-pitched voice, making it difficult to command respect from soldiers and revolutionaries who saw him as effeminate. He was a vegetarian and teetotaler, considered peculiar in Mexico, and an avowed spiritualist. He claimed to have contact with his dead brother Raà ºl and liberal reformer Benito Juarez, who told him to maintain pressure on Dà ­az. Dà ­az Porfirio Dà ­az was an iron-fisted dictator in power since 1876. Dà ­az had modernized the country, laying miles of train tracks and encouraging industry and foreign investment, but at a cost. The poor lived in abject misery. Miners worked without safety measures or insurance, peasants were kicked off their land, and debt peonage meant that thousands were essentially slaves. He was the darling of international investors, who commended him for â€Å"civilizing† an unruly nation. Dà ­az kept tabs on those who opposed him. The regime controlled the press, and rogue journalists could be jailed without trial for libel or sedition. Dà ­az played politicians and military men against one another, leaving few threats to his rule. He appointed all state governors, who shared the spoils of his crooked but lucrative system. Elections were rigged and only the foolish tried to buck the system. Dà ­az had fought off many challenges, but by 1910 cracks were showing. He was in his late 70s, and the wealthy class he represented worried about his successor. Years of repression meant the rural poor and urban working class loathed Dà ­az and were primed for revolution. A revolt by Cananea copper miners in 1906 in Sonora had to be brutally suppressed, showing Mexico and the world that Diaz was vulnerable. 1910 Elections Dà ­az had promised free elections in 1910. Taking him at his word, Madero organized the Anti-Re-Electionist Party to challenge Diaz and published a bestselling book titled  The Presidential Succession of 1910. Part of Maderos platform was that when Dà ­az came to power in 1876, he claimed he wouldnt seek re-election. Madero insisted that no good came from one man holding absolute power and listed Dà ­azs shortcomings, including the massacre of Maya Indians in the Yucatan, the crooked system of governors, and the Cananea mine incident. Mexicans flocked to see Madero and hear his speeches. He began publishing a newspaper,  El Anti-Re-Electionista, and secured his partys nomination. When it became clear that Madero would win, Dà ­az had most of the Anti-Re-Electionist leaders jailed, including Madero, arrested on a false charge of plotting armed insurrection. Because Madero came from a wealthy, well-connected family, Dà ­az could not simply kill him, as he had two generals who had threatened to run against him in 1910. The election was a sham and Dà ­az â€Å"won.†Ã‚  Madero, bailed out of jail by his wealthy father, crossed the border and set up shop in San Antonio, Texas. He declared the election null and void in his â€Å"Plan of San Luà ­s Potosà ­Ã¢â‚¬  and called for armed revolution. November 20 was set for the revolution to begin. Revolution With Madero in revolt, Dà ­az rounded up and killed many of his supporters. The call to revolution was heeded by many Mexicans. In the state of Morelos,  Emiliano Zapata  raised an army of peasants and harassed wealthy landowners. In the state of Chihuahua,  Pascual Orozco  and  Casulo  Herrera raised sizable armies. One of Herreras captains was ruthless revolutionary  Pancho Villa, who replaced the cautious Herrera and, with Orozco, captured cities in Chihuahua in the name of the revolution. In  February 1911, Madero returned from the U.S. Northern leaders including Villa and Orozco didnt trust him, so in March, his force swollen to 600, Madero led an attack on the federal garrison at Casas Grandes, which was a fiasco. Outgunned, Madero and his men retreated, and Madero was injured. Although it ended badly, Maderos bravery gained him respect among the northern rebels. Orozco, at that time leader of the most powerful rebel army, acknowledged Madero as leader of the revolution. Not long after the battle, Madero met  Villa  and they hit it off despite their differences. Villa knew he was a good bandit and rebel chief, but he was no visionary or politician. Madero  was a man of words, not action, and he considered Villa a Robin Hood,  just the man to oust Dà ­az. Madero allowed his men to join Villas force: His days of soldiering were done. Villa and Orozco pushed toward  Mexico City, scoring victories over federal forces along the way. In the south, Zapatas peasant army was capturing towns in his native state of Morelos, beating superior federal forces with a combination of determination and numbers. In May 1911, Zapata scored a huge, bloody victory over federal forces in the town of Cuautla. Dà ­az could see that his rule was crumbling. Dà ­az Quits Dà ­az negotiated a surrender with Madero, who generously allowed the former dictator to leave the country that month. Madero was greeted as a hero when he rode into Mexico City on June 7, 1911. Once he arrived, however, he made a series of mistakes. As interim president, he accepted Francisco Leà ³n de la Barra, a former Dà ­az crony who coalesced the anti-Madero movement. He also demobilized Orozcos and Villas armies. Maderos Presidency Madero became president in November 1911. Never a true revolutionary, Madero simply felt that Mexico was ready for democracy and Dà ­az should step down. He never intended to carry out radical changes, such as land reform. He spent much of his time as president trying to reassure the privileged class that he wouldnt dismantle the power structure left by Dà ­az. Meanwhile, Zapata, realizing that Madero would never approve real land reform, took up arms again. Leà ³n de la Barra, still interim president and working against Madero, sent  Gen. Victoriano Huerta, a brutal remnant of Dà ­azs regime, to Morelos to contain Zapata. Called back to Mexico City, Huerta began conspiring against Madero. When he became president, Maderos only remaining friend was Villa, whose army was demobilized. Orozco, who hadnt gotten the huge rewards he had expected from Madero, took to the field, and many of his former soldiers joined him. Downfall and Execution The politically naive Madero didnt realize he was surrounded by danger. Huerta was conspiring with American ambassador Henry Lane Wilson to remove Madero, as Fà ©lix Dà ­az, Porfirios nephew, took up arms along with Bernardo Reyes. Although Villa rejoined the fight in favor of Madero, he ended up in a stalemate with Orozco. Madero refused to believe his generals would turn on him. The forces of Fà ©lix Dà ­az entered Mexico City, and a 10-day standoff known as la  decena  trgica (â€Å"the tragic fortnight†) ensued. Accepting Huertas â€Å"protection,† Madero fell into his trap: He was arrested by Huerta on Feb. 18,  1913,  and executed four days later, though Huerta said he was killed when his supporters tried to free him. With Madero gone, Huerta turned on his fellow conspirators and made himself president. Legacy Although he wasnt a radical,  Francisco Madero  was the spark that set off the  Mexican Revolution. He was clever, rich, well-connected, and charismatic enough to get the ball rolling against a weakened Porfirio Dà ­az, but couldnt hold onto power once he attained it. The Mexican Revolution was fought by brutal, ruthless men, and the idealistic  Madero  was out of his depth. Still, his name became a rallying cry, especially for Villa and his men. Villa was disappointed that Madero had failed and spent the rest of the revolution looking for another politician to entrust with the future of his country. Maderos brothers were among Villas staunchest supporters. Later politicians tried and failed to unite the nation until 1920, when Alvaro Obregà ³n seized power, the first to succeed at imposing his will on the unruly factions. Decades later, Madero is seen as a hero by Mexicans, the father of the revolution that did much to level the playing field between rich and poor. He is seen as weak but idealistic, an honest, decent man destroyed by the demons he helped to unleash. He was executed before the bloodiest years of the revolution, so his image is unsullied by later events. Sources McLynn, Frank.  Villa and Zapata: A History of the Mexican Revolution.  Basic Books, 2000.Francisco Madero: President of Mexico. Encyclopedia Brittanica.Francisco Madero. Biography.com.

Response to the Discussion by Tamika Case Study Example | Topics and Well Written Essays - 500 words

Response to the Discussion by Tamika - Case Study Example It is essential to develop trust and partnership with the wife of the patient for strengthening and supporting that family through expected and unexpected life events. Therefore, it is important to help Maureen in supporting the interest of the family and she should be given the right guidelines regarding cancer communication. "The family is a significant factor in the health and well-being of individuals, and promotion, maintenance, and restoration of families are important to society's survival." (Registered Nurses Association of Ontario, 2006). Thus, as a nursing practitioner, I would help the wife of the patient to understand the relevance of effective cancer communication and I will collaborate with her in determining the most impeccable resolution regarding this communication. I will convince Maureen not to rush to any futile conclusions and that we will support her in every possible way to offer the best solution to the issue. Thus, after the collaborative discussions and eval uations of the possible courses of actions and their consequences, I will help Maureen to take the right decision regarding the information being withheld from her husband. In her discussion of the specific case provided, Tamika makes some essential points regarding effective cancer communication to the patient and the issues related. At the very start of the discussion, Tamika mentions the possibility of the errors in the decision of Maureen to withhold the information from the patient, while she accepts the situations leading to the wife’s decision.  Ã‚  

Whole food study case Example | Topics and Well Written Essays - 250 words

Whole food - Case Study Example gh it recovered and has started increasing its profits from an average of $300 million to $500 million, it has yet to clear its debts of over $700 million which were accumulated during the period mentioned above. There are several ways to solve this problem and ensure that even if the economy plunges again, they will have no debts accumulating on top of what they have now. The first way is to dispose of some of their assets which are not helping them much through selling them. This is bound to bring in a bit of money to offset the debt. The other way is to increase the number of shares to the public and this will raise money. It can also increase gradually the prices of their commodities especially now that people like the natural and organic products they sell. Even if the money will not repay all the debt, it will at least offset a large sum of money and the rest can be paid off slowly through the proceeds from the company. Thompson, Arthur, Margaret Peteraf, John Gamble and A. J. Strickland. Crafting & Executing Strategy: The Quest for Competitive Advantage: Concepts and Cases. New York: McGraw-Hill Education, 2011.

Healthy life Essay Example for Free

Healthy life Essay Many of the views associated with a healthy lifestyle, but it is not appropriate that we take for granted about this healthy lifestyle . 1 . Caring for Daily Nutrition ( menJaga keseimbangan makanan) Among healthy lifestyle is keeping our daily diet . Therefore, we should adopt a balanced diet . Balanced diet allows our bodies healthy and not overweight. Poor diet causes our body to get a variety of diseases such as hypertension , heart disease and diabetes. Exercise or Play ( bersenam atau beriadah) In addition, we also must diligently work or play. By doing physical activity , the heart will be healthy and strong. Toxic waste in the body will come out through our sweat during exercise . Exercise we are doing will allow the blood to flow properly and strengthen the muscles of the body . 3 . Environmental Cleanliness and Environment Home Practice a healthy lifestyle is keeping the house clean next and the environment. Places that allow mosquitoes to breed must be eliminated. All refuse shall be dubuang to Famous Spots: Yoy reserved . Do not throw garbage into the river or dibaar outdoors because it can cause environmental pollution . 4 . Health Inspection (pemeriksaan kesihatan) In addition, we need to make health checks. Health checks can be made public or private hospital . By knowing our health , we can already taken measures to ensure our health continues to be in good keaaan . Checks can be made two one-year time . 5 . Avoid Doing Affect Health We must also avoid actions that could affect health. Among the things that can affect health is cigarette smoking , alcohol and drugs. The habit of smoking cigarettes can ause a lung disease and heart disease. While drug addiction can lead to social and community causes disease , an obstacle to the progress and development of the country , so let us increase our awareness and understanding of the dangers of drugs , and trying to control and prevent the occurrence of these adverse events . In conclusion , there are some practices that we need to do to enable us to live a healthy life . Practice a healthy lifestyle should be nurtured since we were little where parents and family members to play their role in a healthy lifestyle . So prevention is better than cure. healthy life.

Flow Cytometry for the Evaluation of Semen

Flow Cytometry for the Evaluation of Semen State of the Art in Sperm Assessment Using Flow Cytometry Abstract Flow cytometry is emerging as a substantial tool in the domain of modern andrology for the routine analysis of spermatozoa. Recent application of flow cytometry in the artificial insemination industry especially for pig is a new approach. Until very recent, analysis of semen samples was routinely performed by microscopical evaluation and manual techniques by laboratory operators; analysis is inclined due to comprehensive variability among observers, influencing its clinical validity. During last decade, to evaluate farm animal semen, variety of new flow cytometric techniques have been intercalated which made possible a wide spread evaluation of several sperm functionality and characteristics. Here in this paper, an initiative has been taken to explore numerous current flow cytometry developments pressing for andrological tests. After the invention of flow cytometry, sperm evaluation by traditional (microscopic) means became questioned and avoided due to the robust advantages of flow cytometry over the microscopic methods. By the recent development of diverse fluroscence probes, flow cytometry became capable of analyzing number of sperm characteristics like viability, capacitation, acrosomal integrity, membrane permeability, membrane integrity, mitochondrial status, DNA integrity, decondensation of DNA and differences between gametes based on sex. The application of flow cytometry to their detection allows increased numbers of spermatozoa to be assessed over a short time-period, provides the opportunity of working with small sample sizes, increases the repeatability of data obtained, removes the subjectivity of evaluation and allows simultaneous assessment of multiple fluorochromes. Thus, flow cytometry is a technique capable of generating significantly novel data and allows the design and execution of exper iments that are not yet possible with any other technique. Nowadays, semen evaluation using laboratory analyses is very meaningful to the artificial insemination industry to provide the most desired quality product to customers. Future development of flow cytometric techniques will permit further advances both in our knowledge and in the improvement of assisted reproduction techniques. In this paper, the main semen attributes that can be analyzed with fluorochromes and adapted for use with a flow cytometer will be reviewed and the relationship of these tests to fertility will be discussed. Introduction Up to now, semen evaluation is considered as the most important laboratory test that has enabled us to identify and predict clear-cut cases of fertility (Jarow et al., 2002), infertility or even of potential sub-fertility (Rodrà ­guez-Martà ­nez, 2007). Determination of the potential fertility of semen sample and, in the long run, of the male from which it has been collected is the ultimate goal of semen evaluations in clinically healthy sires. Now a days, many methods for the estimate the possible fertilizing capacity of a semen sample and, or in the word, of the male (reviewed by Dziuk 1996; Rodrà ­guez-Martà ­nez et al. 1997a; Rodrà ­guez-Martà ­nez and Larsson 1998; Saacke et al. 1998; Larsson and Rodrà ­guez-Martà ­nez 2000; Rodrà ­guez- Martà ­nez 2000, 2003; Popwell and Flowers 2004; Graham and Mocà © 2005; Gillan et al. 2005) are existing. The methods routinely accustomed for evaluation of the quality of a semen sample involved an evaluation of general appearance, volume, pH, sperm concentration, viability, morphology and motility. Most of these evaluations are based on microscopic analyses that only measure relatively a few numbers of spermatozoa within a population. In most of the cases, these are time-consuming; results obtained are controversial and are not translatable. It should also be noted that such conventional techniques are apt to extreme inter-ejaculate variation, even when the laboratory methodology has been standardized. In the wake of this information, new opportunities have arisen for the development of methods for the diagnosis of male infertility, many of which have been shown to exhibit a prognostic value that eludes conventional semen profiling. Moreover, ejaculated spermatozoa are nowadays handled for use in assisted reproductive technologies, such as the artificial insemination of chilled, frozen-thawed or sexed semen, and IVF. During this long processes, number of steps like semen extension, fluorophore loading, ultrav iolet and laser illumination, high-speed sorting, cooling and cryopreservation are followed, which create a scope to impose different degrees of change in sperm functionality followed by suffer of damage to sperm membranes, organelles or the DNA content. Therefore, although several assays have been developed to monitor these sperm parameters, recently it is being claimed by many groups that buck of those so-called procedures are incomplete, time consuming and laborious. Flow cytometry in diverse technical applications proposes many advantages for the analysis of sperm quality. Flow cytometry is a method where multiple fluorescences and light scattering can be induced allowing single cell or particles illumination in suspension while they flow very rapidly through a sensing area. The increasing use over the past decade of flow cytometry in the leading laboratories in human and veterinary andrology has dramatically increased our knowledge of sperm function under physiological and biotechnological conditions. Flow cytometers is capable to acquire data from several subpopulations within a sample in a few minutes, making it perfect for assessing heterogenous populations in a semen sample. Flow cytometry was initially developed in the 1960s, after that flow cytometry is performing automated separation of cells based on the unique recognition of cellular patterns in a population feasible (Hulett et al., 1969). Likewise, cellular patterns can be recognized by utilizing such a separation approach, in each cells within a population (Baumgarth and Roederer, 2000; Herzenberg et al., 2006). The first notion of flow cytometry development was for medical and clinical applications such as haematology and oncology. Although still much research is going on these medical areas and account for the vast majority of publications on this robust technique, but during the past few years it is being used in a diverse areas, such as bioprocess monitoring, pharmacology, toxicology, environmental sciences, bacteriology and virology. Together with elevated use in many areas, recent advancement of flow cytometry increased its application in the reproductive biology especially for andrology. Although flow cytometry may overestimate the population of unlabelled cells (Petrunkina and Harrison, 2009), plethora of research from our group in pig (Pena et al., 2003, 2004, 2005; Spjuth et al., 2007; Fernando et al., 2003; Saravia et al.,2005, 2007,2009; De Ambrogi et al., 2006; ) bull (Bergquist et al., 2007; Nagy et al., 2004; Januskauskas et al., 2003; Bergqvist et al., 2007; Hallap et al., 20 05, 2006;) stallion ( Kavak et al., 2003; Morrell et al., 2008) indicate that newly developed fluorescent stains and techniques of flow cytometry has made possible a more widespread analysis of semen quality at a biochemical, ultrastructural and functional level. Therefore, flow cytometry is the current technical solution for rapid, precisely reproducible assessment of sperm suspensions. In this review we have described potentiality and scope of flow cytometry for the evaluation of semen, and the way in which this technique can be used in clinical applications for andrology based on some of our previous experiences. Definition of flow cytometry The definition of a flow cytometer is ‘an instrument which measures the properties of cells in a flowing stream or ‘an instrument that can measure physical, as well as multi-colour fluorescence properties of cells flowing in a stream. In other word, cytometry is a method which measure physical and chemical attributes of cells or other particles. Such a measurement is made when cells or other particles pass in single file through some sort of measuring apparatus in a stream of fluid. The data obtained can be used to understand and monitor biological processes and develop new methods and strategies for cell detection and quantification. Compared to other traditional analytical tools, where a single value for each attribute is obtained for the whole population, flow cytometry provides data for each and every particle detected. As cells differ in their metabolic or physiological states, flow cytometry allows us not only to detect a particular cell type but also to find different subpopulations according to their structural or physiological parameters. Flow cytometry is a technique for measuring components (cells) and the properties of individual cells in liquid suspension. In essence, suspended cells are brought to a detector, one by one, by means of a flow channel. Fluidic devices under laminar flow define the trajectories and velocities that cells traverse across the detector, and fluorescence, absorbance, and light scattering are among the cell properties that can be detected. Flow sorting allows individual cells to be sorted on the basis of their measured properties, and one to three or more global properties of the cell can be measured. Flow cytometers and cell sorters make use of one or more excitation sources and one or two fluorescent dyes to measure and characterize several thousands of cells per second. Flow cytometry presents objective and precise results (Bunthof et al., 2001; Shleeva et al., 2002), which help to overcome the problems with the manual methods described above. Function and types of flow cytometry A flow cytometer is made of three main systems, fluidics, optics and electronics. ItI It can acquire data on all subpopulations within a sample, making it ideal for assessment of heterogenous population, such as spermatozoa. The adaptation of flow cytometry to sperm assessment came in to function when it was used for measuring their DNA content (Evenson et al., 1980) and its application for analyzing semen has been increased rapidly in last decade. Flow cytometry is now applied for the evaluation semen such as sperm viability, acrosomal integrity, mitochondrial function, capacitation status, membrane fluidity, DNA status and so on. Continuous innovation of new fluorescent stains and techniques facilitated the flow cytometric evaluation of spermatozoa. Flow cytometry allows the observation of physical characteristics, such as cell size, shape and internal complexity, and any component or function of the spermatozoon that can be detected by a fluorochrome or fluorescently labeled compound. The analysis is objective, has a high level of experimental repeatability and has the advantage of being able to work with small sample sizes. Flow cytometry also has the capacity to detect labeling by multiple fluorochromes associated with individual spermatozoa, meaning that more than one sperm attribute can be assessed simultaneously. This feature has an added benefit for semen analysis, as few single sperm parameters show significant correlation with fertility in vivo for semen within the acceptable range of normality (Larsson and Rodriguez-Martinez, 2000) and it is the general statistics that the more sperm parameters can be tested, the more accurate the fertility prediction becomes (Amman and Hammerstedt, 1993). There are two main types of flow cytometers-analysers and sorters are in use. Together with data collection on cells, sorters have the potentiality to sort cells with particular properties (defined by the flow cytometer operator) to extremely high purities. There are also a number of commercial flow cytometers that have been developed for particular analytical requirements. Partec manufacture a Ploidy Analyser and also a Cell Counter Analyser. Optoflow has developed a flow cytometer for the rapid detection, characterization and enumeration of microorganisms. Luminex is developing technology for multiplexed analyte quantitation using a combination of microspheres, flow cytometry and high speed digital processing. Advantages of FC compared to other conventional techniques to explore sperm structure and function Use of authentic assays in the fertility clinic and artificial insemination industries increasing day by day. In this respect, use of flow cytometry might be an important attempt to resolve sustaining problem with so called commonly used manual method for the semen analysis. An additional source of laboratory variation is the low number of sperms analyzed with such techniques. It is worth mentinign here that so called method deal only with few hundred sperm. When we deal with such a few sperm population, there is a possibility that obtained result might not be statistically significant (Russel and Curtis, 1993). The methods which are frequently used are enable to determine sperm concentration (Jorgensen et al., 1997), motility or morphology only (Keel et al., 2002). Objectivity, cell number measured, speed of count and precision are the advantages of flow cytometry to conventional light microscopy techniques (Spano and Evenson, 1993). The technique now a days has been used to determi ne a number of factors including those of acrosome status, membrane integrity, mitochondrial function as well as multiparameter measurement in human (Garrido et al., 2002). Flow cytometry has the ability to analyze thousands of cells in few minutes. In our series of studies, we demonstrated the feasibility and reproducibility of an automated method to evaluate sperm cell type, count, and viability in human boar samples. In our hand, the precision of the flow cytometric analysis is satisfactory in a diverse species (boar, bull, stallion etc), and the observed errors were significantly better than those obtained from the so-called manual methods. Although there are diverse benefits of flow cytometer for the analysis of semen, feasibility of applying flow cytometry sometimes restricted to researcher due to the high outlay and difficulties of operation associated with the requirement of a skilled operator. Further, a flow cytometer is very large and cannot resist shocks associated with movement, and it also requires much space in the laboratory. Whatever may be the limitation, the development of more affordable ‘‘bench-top flow cytometers in recent time raised the potential essentialities to semen analysis. If the further application of flow cytometric analysis is considered further, it might be seen that it is growing popularities as a technique for assessing more than one sperm attribute, simultaneously. Compared to traditional microscopic techniques, flow cytometry analysis is allowing to give a far more simplified and objective method of semen analysis, especially in relation to fertilization with acrosome reactivity potential of spermatozoa (Uhler et al., 1993; Purvis et al., 1990; Carver-Ward et al., 1996). A large number of different techniques to estimate sperm concentration have been reported. In the mid-1990s a series of fixed-depth disposable slides were evaluated as rapid and effective pieces of equipment for the estimate of sperm concentration. Data from a number of preliminary studies proposed that, at least in the 20-mm-depth format, such chambers resulted in a noticeable underestimate of sperm concentration compared to the gold standard (improved Neubauer hemocytometer). According to the World Health Organization that ‘‘such chambers, whilst convenient in that they can be used without dilution of the specimen, might lead to inaccuracy (World Health Organization, 1999). Data from Tomlinson and colleagues indicate that two proprietary disposable slides (Microcell, Conception Technologies, San Diego, Calif; Leja, Leja Products, BV Nieuw- Vennep, The Netherlands) can result in a lower concentrations of sperm compared to the hemocytometer method (Tomlinson et al., 2001) . In contrast, plenty of reports document unacceptable differences between different laboratories and even between different individuals, although fewer studies attempt to address these issues. So, what is wrong? Improvement of semen quality testing has been emphasizing in some reports (Jorgensen et al., 1997; Keel et al., 2000). But due to low number of sperm evaluation by the conventional method results in poor reproducibility. These problems might be overcome when using flow cytometry. The validation of method is a challenge due to its essentiality of having specific, precise, objective, and accurate evaluation to establish a correlation of fertility data or to predict potential of a semen sample accurately (Amann, 1989). In a fertility clinic, precision of data in important as the result of semen analysis is frequently used to manage fertility of a patient and treatment of the unfertile couples. Thus, it is important to take into consideration within and between laboratory variations for successful infertility treatments. Sometimes its a matter of argument that compared to flow cytometry, fluorescent microscopy evaluate â€Å"patterns of fluorescence rather than the fluorescence intensity. Flow cytometer has the lack of ability to discriminate sperm containing a fluorescent marker bound to the equatorial segment or over one of the acrosomal membranes (Parinaud et al., 1993; Mortimer and Camenzind, 1989; Mortimer et al., 1987). Tao et al. (1993) compared flow cytometry and epifluorescent microscopy with various lectins and indicated that there is almost no difference between methodologies for detection of the acrosome reaction. However, it has been argued that lectins do not bind specifically to the acrosomal region of the sperm (Purvis et al., 1990; Holden and Trounson, 1991) and that other binding sites can be easily distinguished by epifluorescence microscopy, whereas flow cytometry identifies the signal from the entire sperm. Additionally, conventional light microscopic semen assessment is increasingly being replaced by fluorescent staining techniques, computer-assisted sperm analysis (CASA) systems, and flow cytometry (PenËÅ"a et al., 2001; Verstegen et al., 2002). Additional advantages over existing techniques are that this approach is faster than the hemacytometer and that cellular debris, fat droplets, and other particulate material in extended semen are not erroneously counted as sperm, as often occurs with electronic cell counters. This method can also be used to determine the number of somatic cells in a semen sample. Application of flow cytometry for sperm count Sperm count is an important predominant factor for the evaluation of sperm fertility potential. Accurate determination of sperm cell concentration is critical especially in AI industry because it provides assurance to customers that straws of extended semen contain the sperm numbers indicated which will help to decide appropriate doze especially for pig. Accuracy of sperm count is a common problem in the andrological laboratories and accurate measure of sperm concentration is particularly important for export in which verification of numbers may be required. Routine sperm counts can help to identify possible processing errors within a specific batch of semen or on a particular day, should those errors occur. As sperm counting procedures become more refined, routine counting can be used to monitor subtle changes in daily semen processing that might affect the number of sperm packaged in a straw. Every time new and more accurate methods for the sperm count determinations are coming and being replaced by the older ones. Some laboratories are trying the Maklerm counting chamber (Se if- Medical, Haifa, Israel) and other improved hemacytometers, such as the MicroCellTM (Fertility Technologies, Inc., Natick, MA); however, these techniques will likely have standard lems similar to those associated with the standard hemacytometers. Although hemacytometers are routinely used for sperm counts, due to the slow process and need for multiple measurements of each sample, the chance of error increase. Freund and Carol (ref) stated that a difference of 20% were not unusual between the determinations by the same technician. Electronic counters provide much more rapid counting, are easier to use, and give more repeatable results among technicians. However, those instruments tend to include in the sperm count any somatic cells present, immature sperm forms, cytoplasmic droplets, debris, and bacteria, thereby inflating the concentration value (Ref). Spectrophotometer is recently being used in the AI industries to assess sperm concentration by determining turbidity of a semen s ample using an instrument previously calibrated for sperm concentration with a hemacytometer or Coulter counter (Ref). The accuracy of this method depends on the methods used for spectrophotometer calibration. Although, sperm concentration can also be determined by spectrophotometrically, the debris present in the raw semen crease problem with misestimation. Sperm number in the frozen thawed semen is difficult to ascertain as most of the extender contain egg yolk particles, fats and other particles which affect measurement of sperm with electric cell counter or spectrophotometers (Evenson et al., 1993). On the other hand flow cytometry created possibilities of a rapid determination of sperm number in a precise form. It is the flow cytometry which can reduce intra-laboratory and inter-laboratory variation and conflict regarding sperm concentration assessment. Computer assisted semen analyzer is robust technique for analyzing sperm movement which can count sperm as well; but such an a nalyzer most of the cases use some counting chamber or hemacytometer which itself can generate error. Although, hemacytometer was originally developed for blood cell counting, its use is now diverse including andrological laboratories for sperm counting. Around two-decade ago flow cytometry was suggested for sperm numbers in straws of cryopreserved bull semen. Christensen et al. (-) observed similar results for sperm count with flow cytometry and hemocytometer for a number of species. Now a day a simultaneous determination of sperm viability and sperm concentration is possible which can avoid the chance of occurring differences between ejaculates leading lack of coordination with field fertility and laboratory analyses. Thus the present technology is more precise which can get rid of variation from handling the sperm sample and variation from pipetting and the analysis itself. Further, Prathalingam et al. (2006) concluded that there is similarities for sperm count result between flow cytometry and two newly approached method (image analysis and fluorescent plate reader) for sperm counting. Though, use of fluorescent plate was emphasized due to low cost and allowing large number of cells counting from a large number of ejaculates. Although flow cytometry has become a valuable instrument for andrological determinations, it is also blamed that sperm concentration by flow cytometry signify a higher value than the real one. The possibility arise might be due to that semen samples often contain some alien materials such as immature germ cells, epithelial cells, blood cells, cytoplasmic droplet, cellular debris etc. In the same way, frozen semen has higher chance to introduce such material as they contain diluents components especially egg yolk particles. These particles and cell debris might have frontal and side light scatter parameters those are similar to spermatozoa. Such sperm-count-overestimation problem arisen in our cases also, especially when we deal with frozen semen. Further it is also claimed that flow cytometry has a tendency to overestimate viable spermatozoa. We are also experienced with such trouble which we guess might be due to that egg particles of extender are considered as viable cell as for it s staining pattern. Our yet to publish data indicate that this problem can be mimic by a centrifugation process and by using low concentration sample for evaluation with flow cytometry. Very recently Petrunkina and Harrison (2009) proposed a mathematical equation for fixing this flow cytometric sperm counting. Thus much research is going on and we hope such discrepancy will completely be resolved near future to get advantage from this robust technology for sperm counting. Flow cytometry for detecting sperm intactness -Viability of spermatozoa The viability of spermatozoa is a key determinant of sperm quality and prerequisite for successful fertilization. Viability of spermatozoa can be assessed by numerous methods, but many are slow and poorly repeatable and subjectively assess only 100 to 200 spermatozoa per ejaculate. Merkies et al. (2000) compared different methods of viability evaluation. They concluded that Eosin-nigrosin overestimate viability while fluorescent microscope and flow cytometry estimate similar trend of viability. Current flow cytometric procedures are able to simultaneously evaluate sperm cell viability together with some other attributes. This method has been successfully used for assessing spermatozoa viability in men (Garner and Johnson, 1995), bulls (Garner et al., 1994; Thomas et al., 1998), boars (Rodrà ­guez-Martà ­nez, 2007; Garner and Johnson, 1995; Garner et al., 1996), rams (Garner and Johnson, 1995), rabbits (Garner and Johnson, 1995), mice (Garner and Johnson, 1995; Songsasen et al., 199 7), poultry and wildfowl (Donoghue et al., 1995; Blanco et al., 2000) and honey bees (Collins and Donoghue, 1999; Collins, 2000) and in fish (Martin Flajshans et al., 2004). Considerable information has accumulated on the use of fluorescent staining protocols for assessing sperm viability (Evenson et al., 1982). The SYBR 14 staining of nucleic acids, especially in the sperm head, was very bright in living sperm. Good agreement was observed between the fluorescent staining method and the standard eosin-nigrosine viability test; the flow cytometric method showed a precision level higher than that of the manual method. One of the first attempts to assess sperm viability utilized rhodamine 123 for determining potentiality of mitochondrial membrane while ethidium bromide for membrane integrity through flow cytometry (Garner et al., 1986). Other combinations that have been used to examine the functional capacity of sperm are carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) (Garner et al., 1988; Watson et al., 1992); carboxydimethylfluorescein diacetate (CMFDA), R123, and PI (Ericsson et al., 1993; Thomas and Garner, 1994); and PI, pisum sativum agglutinin (PSA), and R123 (Graham et al., 1990). The most generally used sperm viability stain combinations is SYBR-14 and PI at present. This stains are now sold commercially as live/dead viability kit. When these two stains are combinely used, the nuclei of viable sperm take fluoresce green and membrane integrity lost cells take red stain. This staining technique has been used in a number of species, including the boar (Garner and Johnson, 1995; Saravia et al.,2005, 2007,2009). Although species differences do exist in the function of spermatozoa, the Live/Dead stain may similarly have no adverse affect on fertilization in the equine, although it remains to be tested in this species. Recently a new instrument (Nucelocounter-SP100) has been introduced to evaluate sperm concentration [11] and viability. Due to the small size and low cost, this instrument has been attracted for field measurements of both concentration and viability. In our hand this instrument was also became useful for the quick measurement of sperm concentration an d viability in stallion (Morrell et al., 2010). Fluorescent probes such as H33258, requiring flow cytometric analysis with a laser that operates in the ultraviolet light range, are less commonly used as this is not a standard feature on the smaller analytical machines. However, one alternative is to use a fluorometer. A fluorometer is a relatively low-cost piece of portable equipment that permits a rapid analysis to be carried out on a sample. Januskauskas et al. (2001) used H33258 to detect nonviable bull spermatozoa by fluorometry and obtained an inverse correlation between the damaged cells per cent and the field fertility. Another option is fluorescent attachments for computer-assisted semen analysis devices. For example, the IDENT fluorescence feature of the Hamilton-Thorne IVOS permits staining with H33258 allowing an assessment of sperm viability to be made along with motility. Fluorochromes used to assess sperm viability by both approach could be utilized in combination with each other. In that case, when CFDA is used combined with PI, three populations of cells as live, which are green; dead, which are red; and a third population which is stained with both and represents dying spermatozoa can be identified. This combination was found useful by Almlid and Johnson (1988) for frozen-thawed boar spermatozoa for monitoring membrane damage at the time of evaluation of various freezing protocols. Further, Harrison and Vickers (1990) also noticed that this combination with a fluorescent microscope is effective indicator of viability of fresh, incubated or cold-shocked spermatozoa in boar and ram. Contrasting to these, Garner et al. (1986) was failed to find a relationship between bull sperm viability and fertility when using combination of CFDA/PI . Flow cytometry for evaluating sperm viability appears to be a precious tool in the AI industry. When a high number of sperm is packed in each insemination dose, the effect of selecting the best ejaculates according to sperm viability has a relatively limited effect. However, sperm viability might be more important when combined with low-dose inseminations. The FACSCount AF flow cytometer also determines sperm concentration accurately and precisely during the same analysis (Christensen et al., 2004a). The combined assessment of sperm viability and concentration appears to be useful in the wake of improving quality control at AI stations. Because of the results of this trial, this method has been implemented by Danish AI stations (Christensen et al., 2005). Relatively bright fluorescence was found also in the mitochondrial sheath of living sperm. But the mechanism and mode of action by which SYBR-14 binds to the DNA of sperm is not known. It is know that PI stains nucleic acids by inte rcalating between the base pairs (Krishan, 1975). Viability stains can also be used in conjugation with fluorescently labeled plant lectins for simultaneous assessment of the plasma membrane integrity and the acrosome integrity (Nagy et al., 2003). It is conceivable that assessment of viability using SYBR-14 dye does not damage spermatozoa, since Garner et al. (5) found that insemination of boar sperm stained with SYBR-14 did not compromise fertilization or even the development of flushed porcine embryos in vitro. Non-viable sperms can be detected using the membrane-impermeable nucleic acid stains which positively identify dead spermatozoa by penetrating cells with damaged membranes. Plasma membrane which is intact will not permit these stains entering into the spermatozoa and staining the nucleus. Most frequently used stains include phenanthridines, for example propidium iodide (PI; (Matyus, 1984) ethidium homodimer-1 (EthD-1; (Althouse et al., 1995), the cyanine Yo-Pro (Kavak, 2003) and the bizbenzimidazole Hoechst 33258 (Gundersen and Shapiro, 1984). After a series of comparison between fertility of cryopreserved stallion spermatozoa with a number of laboratory assessments of semen quality as assessed by flow cytometry using PI, Wilhelm et al. (1996) concluded that viability is the single laboratory assay that correlated with fertility. -Sperm plasma membrane integrity Although the sperm plasma membrane covers the entire cell, it consists of three distinct membrane compartments, one which covers the outer acrosomal membrane, one which covers the post acrosomal portion of the sperm head, and one which covers the middle and principal pieces. Sperm membrane is directly or ind Flow Cytometry for the Evaluation of Semen Flow Cytometry for the Evaluation of Semen State of the Art in Sperm Assessment Using Flow Cytometry Abstract Flow cytometry is emerging as a substantial tool in the domain of modern andrology for the routine analysis of spermatozoa. Recent application of flow cytometry in the artificial insemination industry especially for pig is a new approach. Until very recent, analysis of semen samples was routinely performed by microscopical evaluation and manual techniques by laboratory operators; analysis is inclined due to comprehensive variability among observers, influencing its clinical validity. During last decade, to evaluate farm animal semen, variety of new flow cytometric techniques have been intercalated which made possible a wide spread evaluation of several sperm functionality and characteristics. Here in this paper, an initiative has been taken to explore numerous current flow cytometry developments pressing for andrological tests. After the invention of flow cytometry, sperm evaluation by traditional (microscopic) means became questioned and avoided due to the robust advantages of flow cytometry over the microscopic methods. By the recent development of diverse fluroscence probes, flow cytometry became capable of analyzing number of sperm characteristics like viability, capacitation, acrosomal integrity, membrane permeability, membrane integrity, mitochondrial status, DNA integrity, decondensation of DNA and differences between gametes based on sex. The application of flow cytometry to their detection allows increased numbers of spermatozoa to be assessed over a short time-period, provides the opportunity of working with small sample sizes, increases the repeatability of data obtained, removes the subjectivity of evaluation and allows simultaneous assessment of multiple fluorochromes. Thus, flow cytometry is a technique capable of generating significantly novel data and allows the design and execution of exper iments that are not yet possible with any other technique. Nowadays, semen evaluation using laboratory analyses is very meaningful to the artificial insemination industry to provide the most desired quality product to customers. Future development of flow cytometric techniques will permit further advances both in our knowledge and in the improvement of assisted reproduction techniques. In this paper, the main semen attributes that can be analyzed with fluorochromes and adapted for use with a flow cytometer will be reviewed and the relationship of these tests to fertility will be discussed. Introduction Up to now, semen evaluation is considered as the most important laboratory test that has enabled us to identify and predict clear-cut cases of fertility (Jarow et al., 2002), infertility or even of potential sub-fertility (Rodrà ­guez-Martà ­nez, 2007). Determination of the potential fertility of semen sample and, in the long run, of the male from which it has been collected is the ultimate goal of semen evaluations in clinically healthy sires. Now a days, many methods for the estimate the possible fertilizing capacity of a semen sample and, or in the word, of the male (reviewed by Dziuk 1996; Rodrà ­guez-Martà ­nez et al. 1997a; Rodrà ­guez-Martà ­nez and Larsson 1998; Saacke et al. 1998; Larsson and Rodrà ­guez-Martà ­nez 2000; Rodrà ­guez- Martà ­nez 2000, 2003; Popwell and Flowers 2004; Graham and Mocà © 2005; Gillan et al. 2005) are existing. The methods routinely accustomed for evaluation of the quality of a semen sample involved an evaluation of general appearance, volume, pH, sperm concentration, viability, morphology and motility. Most of these evaluations are based on microscopic analyses that only measure relatively a few numbers of spermatozoa within a population. In most of the cases, these are time-consuming; results obtained are controversial and are not translatable. It should also be noted that such conventional techniques are apt to extreme inter-ejaculate variation, even when the laboratory methodology has been standardized. In the wake of this information, new opportunities have arisen for the development of methods for the diagnosis of male infertility, many of which have been shown to exhibit a prognostic value that eludes conventional semen profiling. Moreover, ejaculated spermatozoa are nowadays handled for use in assisted reproductive technologies, such as the artificial insemination of chilled, frozen-thawed or sexed semen, and IVF. During this long processes, number of steps like semen extension, fluorophore loading, ultrav iolet and laser illumination, high-speed sorting, cooling and cryopreservation are followed, which create a scope to impose different degrees of change in sperm functionality followed by suffer of damage to sperm membranes, organelles or the DNA content. Therefore, although several assays have been developed to monitor these sperm parameters, recently it is being claimed by many groups that buck of those so-called procedures are incomplete, time consuming and laborious. Flow cytometry in diverse technical applications proposes many advantages for the analysis of sperm quality. Flow cytometry is a method where multiple fluorescences and light scattering can be induced allowing single cell or particles illumination in suspension while they flow very rapidly through a sensing area. The increasing use over the past decade of flow cytometry in the leading laboratories in human and veterinary andrology has dramatically increased our knowledge of sperm function under physiological and biotechnological conditions. Flow cytometers is capable to acquire data from several subpopulations within a sample in a few minutes, making it perfect for assessing heterogenous populations in a semen sample. Flow cytometry was initially developed in the 1960s, after that flow cytometry is performing automated separation of cells based on the unique recognition of cellular patterns in a population feasible (Hulett et al., 1969). Likewise, cellular patterns can be recognized by utilizing such a separation approach, in each cells within a population (Baumgarth and Roederer, 2000; Herzenberg et al., 2006). The first notion of flow cytometry development was for medical and clinical applications such as haematology and oncology. Although still much research is going on these medical areas and account for the vast majority of publications on this robust technique, but during the past few years it is being used in a diverse areas, such as bioprocess monitoring, pharmacology, toxicology, environmental sciences, bacteriology and virology. Together with elevated use in many areas, recent advancement of flow cytometry increased its application in the reproductive biology especially for andrology. Although flow cytometry may overestimate the population of unlabelled cells (Petrunkina and Harrison, 2009), plethora of research from our group in pig (Pena et al., 2003, 2004, 2005; Spjuth et al., 2007; Fernando et al., 2003; Saravia et al.,2005, 2007,2009; De Ambrogi et al., 2006; ) bull (Bergquist et al., 2007; Nagy et al., 2004; Januskauskas et al., 2003; Bergqvist et al., 2007; Hallap et al., 20 05, 2006;) stallion ( Kavak et al., 2003; Morrell et al., 2008) indicate that newly developed fluorescent stains and techniques of flow cytometry has made possible a more widespread analysis of semen quality at a biochemical, ultrastructural and functional level. Therefore, flow cytometry is the current technical solution for rapid, precisely reproducible assessment of sperm suspensions. In this review we have described potentiality and scope of flow cytometry for the evaluation of semen, and the way in which this technique can be used in clinical applications for andrology based on some of our previous experiences. Definition of flow cytometry The definition of a flow cytometer is ‘an instrument which measures the properties of cells in a flowing stream or ‘an instrument that can measure physical, as well as multi-colour fluorescence properties of cells flowing in a stream. In other word, cytometry is a method which measure physical and chemical attributes of cells or other particles. Such a measurement is made when cells or other particles pass in single file through some sort of measuring apparatus in a stream of fluid. The data obtained can be used to understand and monitor biological processes and develop new methods and strategies for cell detection and quantification. Compared to other traditional analytical tools, where a single value for each attribute is obtained for the whole population, flow cytometry provides data for each and every particle detected. As cells differ in their metabolic or physiological states, flow cytometry allows us not only to detect a particular cell type but also to find different subpopulations according to their structural or physiological parameters. Flow cytometry is a technique for measuring components (cells) and the properties of individual cells in liquid suspension. In essence, suspended cells are brought to a detector, one by one, by means of a flow channel. Fluidic devices under laminar flow define the trajectories and velocities that cells traverse across the detector, and fluorescence, absorbance, and light scattering are among the cell properties that can be detected. Flow sorting allows individual cells to be sorted on the basis of their measured properties, and one to three or more global properties of the cell can be measured. Flow cytometers and cell sorters make use of one or more excitation sources and one or two fluorescent dyes to measure and characterize several thousands of cells per second. Flow cytometry presents objective and precise results (Bunthof et al., 2001; Shleeva et al., 2002), which help to overcome the problems with the manual methods described above. Function and types of flow cytometry A flow cytometer is made of three main systems, fluidics, optics and electronics. ItI It can acquire data on all subpopulations within a sample, making it ideal for assessment of heterogenous population, such as spermatozoa. The adaptation of flow cytometry to sperm assessment came in to function when it was used for measuring their DNA content (Evenson et al., 1980) and its application for analyzing semen has been increased rapidly in last decade. Flow cytometry is now applied for the evaluation semen such as sperm viability, acrosomal integrity, mitochondrial function, capacitation status, membrane fluidity, DNA status and so on. Continuous innovation of new fluorescent stains and techniques facilitated the flow cytometric evaluation of spermatozoa. Flow cytometry allows the observation of physical characteristics, such as cell size, shape and internal complexity, and any component or function of the spermatozoon that can be detected by a fluorochrome or fluorescently labeled compound. The analysis is objective, has a high level of experimental repeatability and has the advantage of being able to work with small sample sizes. Flow cytometry also has the capacity to detect labeling by multiple fluorochromes associated with individual spermatozoa, meaning that more than one sperm attribute can be assessed simultaneously. This feature has an added benefit for semen analysis, as few single sperm parameters show significant correlation with fertility in vivo for semen within the acceptable range of normality (Larsson and Rodriguez-Martinez, 2000) and it is the general statistics that the more sperm parameters can be tested, the more accurate the fertility prediction becomes (Amman and Hammerstedt, 1993). There are two main types of flow cytometers-analysers and sorters are in use. Together with data collection on cells, sorters have the potentiality to sort cells with particular properties (defined by the flow cytometer operator) to extremely high purities. There are also a number of commercial flow cytometers that have been developed for particular analytical requirements. Partec manufacture a Ploidy Analyser and also a Cell Counter Analyser. Optoflow has developed a flow cytometer for the rapid detection, characterization and enumeration of microorganisms. Luminex is developing technology for multiplexed analyte quantitation using a combination of microspheres, flow cytometry and high speed digital processing. Advantages of FC compared to other conventional techniques to explore sperm structure and function Use of authentic assays in the fertility clinic and artificial insemination industries increasing day by day. In this respect, use of flow cytometry might be an important attempt to resolve sustaining problem with so called commonly used manual method for the semen analysis. An additional source of laboratory variation is the low number of sperms analyzed with such techniques. It is worth mentinign here that so called method deal only with few hundred sperm. When we deal with such a few sperm population, there is a possibility that obtained result might not be statistically significant (Russel and Curtis, 1993). The methods which are frequently used are enable to determine sperm concentration (Jorgensen et al., 1997), motility or morphology only (Keel et al., 2002). Objectivity, cell number measured, speed of count and precision are the advantages of flow cytometry to conventional light microscopy techniques (Spano and Evenson, 1993). The technique now a days has been used to determi ne a number of factors including those of acrosome status, membrane integrity, mitochondrial function as well as multiparameter measurement in human (Garrido et al., 2002). Flow cytometry has the ability to analyze thousands of cells in few minutes. In our series of studies, we demonstrated the feasibility and reproducibility of an automated method to evaluate sperm cell type, count, and viability in human boar samples. In our hand, the precision of the flow cytometric analysis is satisfactory in a diverse species (boar, bull, stallion etc), and the observed errors were significantly better than those obtained from the so-called manual methods. Although there are diverse benefits of flow cytometer for the analysis of semen, feasibility of applying flow cytometry sometimes restricted to researcher due to the high outlay and difficulties of operation associated with the requirement of a skilled operator. Further, a flow cytometer is very large and cannot resist shocks associated with movement, and it also requires much space in the laboratory. Whatever may be the limitation, the development of more affordable ‘‘bench-top flow cytometers in recent time raised the potential essentialities to semen analysis. If the further application of flow cytometric analysis is considered further, it might be seen that it is growing popularities as a technique for assessing more than one sperm attribute, simultaneously. Compared to traditional microscopic techniques, flow cytometry analysis is allowing to give a far more simplified and objective method of semen analysis, especially in relation to fertilization with acrosome reactivity potential of spermatozoa (Uhler et al., 1993; Purvis et al., 1990; Carver-Ward et al., 1996). A large number of different techniques to estimate sperm concentration have been reported. In the mid-1990s a series of fixed-depth disposable slides were evaluated as rapid and effective pieces of equipment for the estimate of sperm concentration. Data from a number of preliminary studies proposed that, at least in the 20-mm-depth format, such chambers resulted in a noticeable underestimate of sperm concentration compared to the gold standard (improved Neubauer hemocytometer). According to the World Health Organization that ‘‘such chambers, whilst convenient in that they can be used without dilution of the specimen, might lead to inaccuracy (World Health Organization, 1999). Data from Tomlinson and colleagues indicate that two proprietary disposable slides (Microcell, Conception Technologies, San Diego, Calif; Leja, Leja Products, BV Nieuw- Vennep, The Netherlands) can result in a lower concentrations of sperm compared to the hemocytometer method (Tomlinson et al., 2001) . In contrast, plenty of reports document unacceptable differences between different laboratories and even between different individuals, although fewer studies attempt to address these issues. So, what is wrong? Improvement of semen quality testing has been emphasizing in some reports (Jorgensen et al., 1997; Keel et al., 2000). But due to low number of sperm evaluation by the conventional method results in poor reproducibility. These problems might be overcome when using flow cytometry. The validation of method is a challenge due to its essentiality of having specific, precise, objective, and accurate evaluation to establish a correlation of fertility data or to predict potential of a semen sample accurately (Amann, 1989). In a fertility clinic, precision of data in important as the result of semen analysis is frequently used to manage fertility of a patient and treatment of the unfertile couples. Thus, it is important to take into consideration within and between laboratory variations for successful infertility treatments. Sometimes its a matter of argument that compared to flow cytometry, fluorescent microscopy evaluate â€Å"patterns of fluorescence rather than the fluorescence intensity. Flow cytometer has the lack of ability to discriminate sperm containing a fluorescent marker bound to the equatorial segment or over one of the acrosomal membranes (Parinaud et al., 1993; Mortimer and Camenzind, 1989; Mortimer et al., 1987). Tao et al. (1993) compared flow cytometry and epifluorescent microscopy with various lectins and indicated that there is almost no difference between methodologies for detection of the acrosome reaction. However, it has been argued that lectins do not bind specifically to the acrosomal region of the sperm (Purvis et al., 1990; Holden and Trounson, 1991) and that other binding sites can be easily distinguished by epifluorescence microscopy, whereas flow cytometry identifies the signal from the entire sperm. Additionally, conventional light microscopic semen assessment is increasingly being replaced by fluorescent staining techniques, computer-assisted sperm analysis (CASA) systems, and flow cytometry (PenËÅ"a et al., 2001; Verstegen et al., 2002). Additional advantages over existing techniques are that this approach is faster than the hemacytometer and that cellular debris, fat droplets, and other particulate material in extended semen are not erroneously counted as sperm, as often occurs with electronic cell counters. This method can also be used to determine the number of somatic cells in a semen sample. Application of flow cytometry for sperm count Sperm count is an important predominant factor for the evaluation of sperm fertility potential. Accurate determination of sperm cell concentration is critical especially in AI industry because it provides assurance to customers that straws of extended semen contain the sperm numbers indicated which will help to decide appropriate doze especially for pig. Accuracy of sperm count is a common problem in the andrological laboratories and accurate measure of sperm concentration is particularly important for export in which verification of numbers may be required. Routine sperm counts can help to identify possible processing errors within a specific batch of semen or on a particular day, should those errors occur. As sperm counting procedures become more refined, routine counting can be used to monitor subtle changes in daily semen processing that might affect the number of sperm packaged in a straw. Every time new and more accurate methods for the sperm count determinations are coming and being replaced by the older ones. Some laboratories are trying the Maklerm counting chamber (Se if- Medical, Haifa, Israel) and other improved hemacytometers, such as the MicroCellTM (Fertility Technologies, Inc., Natick, MA); however, these techniques will likely have standard lems similar to those associated with the standard hemacytometers. Although hemacytometers are routinely used for sperm counts, due to the slow process and need for multiple measurements of each sample, the chance of error increase. Freund and Carol (ref) stated that a difference of 20% were not unusual between the determinations by the same technician. Electronic counters provide much more rapid counting, are easier to use, and give more repeatable results among technicians. However, those instruments tend to include in the sperm count any somatic cells present, immature sperm forms, cytoplasmic droplets, debris, and bacteria, thereby inflating the concentration value (Ref). Spectrophotometer is recently being used in the AI industries to assess sperm concentration by determining turbidity of a semen s ample using an instrument previously calibrated for sperm concentration with a hemacytometer or Coulter counter (Ref). The accuracy of this method depends on the methods used for spectrophotometer calibration. Although, sperm concentration can also be determined by spectrophotometrically, the debris present in the raw semen crease problem with misestimation. Sperm number in the frozen thawed semen is difficult to ascertain as most of the extender contain egg yolk particles, fats and other particles which affect measurement of sperm with electric cell counter or spectrophotometers (Evenson et al., 1993). On the other hand flow cytometry created possibilities of a rapid determination of sperm number in a precise form. It is the flow cytometry which can reduce intra-laboratory and inter-laboratory variation and conflict regarding sperm concentration assessment. Computer assisted semen analyzer is robust technique for analyzing sperm movement which can count sperm as well; but such an a nalyzer most of the cases use some counting chamber or hemacytometer which itself can generate error. Although, hemacytometer was originally developed for blood cell counting, its use is now diverse including andrological laboratories for sperm counting. Around two-decade ago flow cytometry was suggested for sperm numbers in straws of cryopreserved bull semen. Christensen et al. (-) observed similar results for sperm count with flow cytometry and hemocytometer for a number of species. Now a day a simultaneous determination of sperm viability and sperm concentration is possible which can avoid the chance of occurring differences between ejaculates leading lack of coordination with field fertility and laboratory analyses. Thus the present technology is more precise which can get rid of variation from handling the sperm sample and variation from pipetting and the analysis itself. Further, Prathalingam et al. (2006) concluded that there is similarities for sperm count result between flow cytometry and two newly approached method (image analysis and fluorescent plate reader) for sperm counting. Though, use of fluorescent plate was emphasized due to low cost and allowing large number of cells counting from a large number of ejaculates. Although flow cytometry has become a valuable instrument for andrological determinations, it is also blamed that sperm concentration by flow cytometry signify a higher value than the real one. The possibility arise might be due to that semen samples often contain some alien materials such as immature germ cells, epithelial cells, blood cells, cytoplasmic droplet, cellular debris etc. In the same way, frozen semen has higher chance to introduce such material as they contain diluents components especially egg yolk particles. These particles and cell debris might have frontal and side light scatter parameters those are similar to spermatozoa. Such sperm-count-overestimation problem arisen in our cases also, especially when we deal with frozen semen. Further it is also claimed that flow cytometry has a tendency to overestimate viable spermatozoa. We are also experienced with such trouble which we guess might be due to that egg particles of extender are considered as viable cell as for it s staining pattern. Our yet to publish data indicate that this problem can be mimic by a centrifugation process and by using low concentration sample for evaluation with flow cytometry. Very recently Petrunkina and Harrison (2009) proposed a mathematical equation for fixing this flow cytometric sperm counting. Thus much research is going on and we hope such discrepancy will completely be resolved near future to get advantage from this robust technology for sperm counting. Flow cytometry for detecting sperm intactness -Viability of spermatozoa The viability of spermatozoa is a key determinant of sperm quality and prerequisite for successful fertilization. Viability of spermatozoa can be assessed by numerous methods, but many are slow and poorly repeatable and subjectively assess only 100 to 200 spermatozoa per ejaculate. Merkies et al. (2000) compared different methods of viability evaluation. They concluded that Eosin-nigrosin overestimate viability while fluorescent microscope and flow cytometry estimate similar trend of viability. Current flow cytometric procedures are able to simultaneously evaluate sperm cell viability together with some other attributes. This method has been successfully used for assessing spermatozoa viability in men (Garner and Johnson, 1995), bulls (Garner et al., 1994; Thomas et al., 1998), boars (Rodrà ­guez-Martà ­nez, 2007; Garner and Johnson, 1995; Garner et al., 1996), rams (Garner and Johnson, 1995), rabbits (Garner and Johnson, 1995), mice (Garner and Johnson, 1995; Songsasen et al., 199 7), poultry and wildfowl (Donoghue et al., 1995; Blanco et al., 2000) and honey bees (Collins and Donoghue, 1999; Collins, 2000) and in fish (Martin Flajshans et al., 2004). Considerable information has accumulated on the use of fluorescent staining protocols for assessing sperm viability (Evenson et al., 1982). The SYBR 14 staining of nucleic acids, especially in the sperm head, was very bright in living sperm. Good agreement was observed between the fluorescent staining method and the standard eosin-nigrosine viability test; the flow cytometric method showed a precision level higher than that of the manual method. One of the first attempts to assess sperm viability utilized rhodamine 123 for determining potentiality of mitochondrial membrane while ethidium bromide for membrane integrity through flow cytometry (Garner et al., 1986). Other combinations that have been used to examine the functional capacity of sperm are carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) (Garner et al., 1988; Watson et al., 1992); carboxydimethylfluorescein diacetate (CMFDA), R123, and PI (Ericsson et al., 1993; Thomas and Garner, 1994); and PI, pisum sativum agglutinin (PSA), and R123 (Graham et al., 1990). The most generally used sperm viability stain combinations is SYBR-14 and PI at present. This stains are now sold commercially as live/dead viability kit. When these two stains are combinely used, the nuclei of viable sperm take fluoresce green and membrane integrity lost cells take red stain. This staining technique has been used in a number of species, including the boar (Garner and Johnson, 1995; Saravia et al.,2005, 2007,2009). Although species differences do exist in the function of spermatozoa, the Live/Dead stain may similarly have no adverse affect on fertilization in the equine, although it remains to be tested in this species. Recently a new instrument (Nucelocounter-SP100) has been introduced to evaluate sperm concentration [11] and viability. Due to the small size and low cost, this instrument has been attracted for field measurements of both concentration and viability. In our hand this instrument was also became useful for the quick measurement of sperm concentration an d viability in stallion (Morrell et al., 2010). Fluorescent probes such as H33258, requiring flow cytometric analysis with a laser that operates in the ultraviolet light range, are less commonly used as this is not a standard feature on the smaller analytical machines. However, one alternative is to use a fluorometer. A fluorometer is a relatively low-cost piece of portable equipment that permits a rapid analysis to be carried out on a sample. Januskauskas et al. (2001) used H33258 to detect nonviable bull spermatozoa by fluorometry and obtained an inverse correlation between the damaged cells per cent and the field fertility. Another option is fluorescent attachments for computer-assisted semen analysis devices. For example, the IDENT fluorescence feature of the Hamilton-Thorne IVOS permits staining with H33258 allowing an assessment of sperm viability to be made along with motility. Fluorochromes used to assess sperm viability by both approach could be utilized in combination with each other. In that case, when CFDA is used combined with PI, three populations of cells as live, which are green; dead, which are red; and a third population which is stained with both and represents dying spermatozoa can be identified. This combination was found useful by Almlid and Johnson (1988) for frozen-thawed boar spermatozoa for monitoring membrane damage at the time of evaluation of various freezing protocols. Further, Harrison and Vickers (1990) also noticed that this combination with a fluorescent microscope is effective indicator of viability of fresh, incubated or cold-shocked spermatozoa in boar and ram. Contrasting to these, Garner et al. (1986) was failed to find a relationship between bull sperm viability and fertility when using combination of CFDA/PI . Flow cytometry for evaluating sperm viability appears to be a precious tool in the AI industry. When a high number of sperm is packed in each insemination dose, the effect of selecting the best ejaculates according to sperm viability has a relatively limited effect. However, sperm viability might be more important when combined with low-dose inseminations. The FACSCount AF flow cytometer also determines sperm concentration accurately and precisely during the same analysis (Christensen et al., 2004a). The combined assessment of sperm viability and concentration appears to be useful in the wake of improving quality control at AI stations. Because of the results of this trial, this method has been implemented by Danish AI stations (Christensen et al., 2005). Relatively bright fluorescence was found also in the mitochondrial sheath of living sperm. But the mechanism and mode of action by which SYBR-14 binds to the DNA of sperm is not known. It is know that PI stains nucleic acids by inte rcalating between the base pairs (Krishan, 1975). Viability stains can also be used in conjugation with fluorescently labeled plant lectins for simultaneous assessment of the plasma membrane integrity and the acrosome integrity (Nagy et al., 2003). It is conceivable that assessment of viability using SYBR-14 dye does not damage spermatozoa, since Garner et al. (5) found that insemination of boar sperm stained with SYBR-14 did not compromise fertilization or even the development of flushed porcine embryos in vitro. Non-viable sperms can be detected using the membrane-impermeable nucleic acid stains which positively identify dead spermatozoa by penetrating cells with damaged membranes. Plasma membrane which is intact will not permit these stains entering into the spermatozoa and staining the nucleus. Most frequently used stains include phenanthridines, for example propidium iodide (PI; (Matyus, 1984) ethidium homodimer-1 (EthD-1; (Althouse et al., 1995), the cyanine Yo-Pro (Kavak, 2003) and the bizbenzimidazole Hoechst 33258 (Gundersen and Shapiro, 1984). After a series of comparison between fertility of cryopreserved stallion spermatozoa with a number of laboratory assessments of semen quality as assessed by flow cytometry using PI, Wilhelm et al. (1996) concluded that viability is the single laboratory assay that correlated with fertility. -Sperm plasma membrane integrity Although the sperm plasma membrane covers the entire cell, it consists of three distinct membrane compartments, one which covers the outer acrosomal membrane, one which covers the post acrosomal portion of the sperm head, and one which covers the middle and principal pieces. Sperm membrane is directly or ind

Analysis of ”Good People” by David Foster Wallace Essay

Analysis of †Good People† by David Foster Wallace, 2007 The short story is set at a park by a lake. â€Å"They were up on a picnic table at that park by the lake, by the edge of the lake, with part of a downed tree in the shallows half hidden by the bank.† The downed tree sets the mood to be sad and dark. We also learn that the main characters Lane A. Dean, Jr. and his girlfriend Sheri Fisher are sitting very still on the picnic table, which tells us that the atmosphere is quite intense. It does not say for how long they sit by the lake, but it says that the right sides of their faces get shaded so it can be assumed that they sit there for a while. One of the main characters is Lane A. Dean, Jr., who is 19 years old and studies accounting and business. Lane is a very reflective person because he reflects a lot on how he is and how he thinks he should be. â€Å"He knew it was wrong, knew something was required of him that was not this terrible frozen care and cauti on, but he pretended to himself he did not know what it was that was required.† This shows that he is a conscientious person and that he has high expectations for himself. Through the whole short story thoughts like the mentioned quote are seen. It seems like Lane is not satisfied with the way he is and keeps comparing himself to his girlfriend, Sheri. â€Å"He was starting to believe that he might not be serious in his faith. He was desperate to be good people, to still be able to feel he was good.†The fact that he is moving away from his faith makes him question whether he is a good person or not. It makes him confused about his identity, about who he is. Lane is also confused when it comes to his love for his girlfriend and that could be the reason why they are not happy sitting together. Lane’s girlfriend, Sheri, is 20 years old, studies to become a nurse and has a hosting job. She is very serious in her faith and values and she is a girl who knows what she wants.So Sheri seems more secure than Lane and more comfortable in her own skin. In a way Sheri is more mature. But she also sits very still: â€Å"She was blank and hidden.† It is shown that Sheri is not happy because she sits with her face in her hands. So it can be concluded that they may have a problem with each other. It is a third person narrative. The narrator has an obvious focus on Lane and this we see because the narrator only includes Lane’s thoughts and feelings a lot: â€Å"Sometimes when alone and thinking or struggling to turn matter over to Jesus Christ in prayer, he would find himself (†¦)† â€Å"He could almost  visualize himself tiptoeing past something explosive.† Therefore the narrator has an inner view of Lane. We learn his opinions, and especially when Sheri is described – you learn that it is through Lane that she is described. The effect of the inner view is that we only learn how one of the main characters is feeling and is thinking. It also makes the information about Sheri subjective because it is Lane’s opinion about her and not her exact thoughts or feelings. We only have Lane’s opinion and actions and Sheri’s actions. Furthermore the short story is written in the past tense, and there are some flashbacks: â€Å"Two days before (†¦)†Some times when they had prayed (†¦)† The flashbacks can enlighten the reader about the main characters background, but also confuse the reader. The short story consists of both short sentences and long sentences. The sentences are short when something dramatic happens and the short sentences make it more dramatic and interesting to read and it also speeds up the reading pace. For example when Lane describes the battle within himself: â€Å"Two hearted, a hypocrite to yourself either way.† There is no direct speech, which makes it harder for the reader to interpret the characters because the writer influences the readers. The writer is presenting an interpretation of the characters’ speech and not their exact words. There is a focus on actions so there is a lot of verbs and also adjectives, especially in the beginning. There are some difficult words, but all in all the vocabulary is standard level. The main conflict is the conflict between Lane and Sheri. First of all both of them sit very still and we hear a lot of Lane’s thoughts – as if they are not talking to each other. â€Å"It was of two great and terrible armies within himself, opposed and facing each other, silent. There would be battle but no victor.† The battle inside of Lane could represent the battle between Sheri and him. You might say that they have a probl em when it comes to communicating with each other. The conflict is that Lane does not love Sheri. â€Å"(†¦) at the decision together did not ever include it – the word – for had he once said it, avowed that he did love her, loved Sheri Fisher, then it all would have been transformed.†18 â€Å"But neither did he ever open up and tell her straight out he did not love her.† Lane expresses that if he had loved her or said to her that he loved her, they would not have this conflict. The conflict is that Lane is not honest with Sheri about his true feelings and this leads to the unhappy and tense atmosphere. The reason why  Sheri does not say anything could be because she is waiting for Lane to come clean and be honest. At the ending of the story Lane imagines what he wants Sheri to say when he tells her the truth – that he does not love her. Lane wants Sheri to say that it is all right and that she wants the best for him. Lane has been praying for love and at the end he comes to realize that he h as been praying for the wrong thing. He should have been praying for courage – courage to tell Sheri the truth. The title â€Å"Good People† refers to the fact that Lane wants to be a good person by being honest with Sheri. It also refers to when Lane says that he is not that serious in his faith and that he wants to be good people, to feel that he was good. Lane does not want to lie to her or to himself. To be good is something every human being aspires to be. To be good, kind and loving to your family, friends and neighbours is something preached by the Christian church, but it is also how people generally want to be in spite of their religion. Lane finds out at the very end that he is not that serious in his faith, but that he is still able to be good by finding the courage to be honest with Sheri.

Information Flow in an Organization Essay

Depending upon the organization information is used and disseminated accordingly. Information flow plays a very important role, and is a critical component among businesses who seek to be more successful than their competition. Companies cannot operate without a proper and concise information flow which is accessible through the company’s different departments. The IT department and information systems are mainly responsible for providing ways for the various departments within the company to have access to applications and systems that assist employees in accomplishing their jobs with more ease. Businesses today rely heavily on information technology, and software applications to assist the different departments through helping them complete their daily tasks and functions in a faster and better manner. Software applications help the information systems run in a way that allow employees to perform more tasks in less time and help everything run smoothly together similar. As I examine my current employer I can see how important information flow is to the success of our company. Information is used to work together with every department; however it is easy to point out how it flows by examining each department such as Sales, Engineering, Programming and Production. As orders are placed, our sales department is the first step in information flow in my organization. Customers have specific requirements for each machine they purchase which generally always differ from previous versions we have made. Once the sales orders and specifications are in order they are sent from the sales department to our Engineering department to be designed. There are several different draftsmen and each has their own area of expertise. The Production Manager assigns the frames and machines to be designed to the draftsmen based on the specifications given by the customer. Also, while in Engineering, the machines are given job numbers that will follow them throughout the shop so that it may be tracked as well as allow employees to clock into the proper job. Having a specific job number tied to a specific machine also allows management to review every individual that has worked on these machines at any time. After the machines have designed and approved they are then sent to the programming department. The programming department is responsible for tearing the machines apart in a CAD system known as SolidWorks and separating the frame from the sheet metal. After this has been completed the next step is to individually program each piece of tubing in the frame and apply the proper programming required for optimum cutting time on the laser. The next thing that is programmed is all the sheet metal parts. In order to program sheet metal, the part which is drawn in solidworks, must be saved and transformed into a DXF file. These files are put into a software called SigmaNest and programmed fairly easy. Once all elements of the machine has been programmed, the programming department takes the programs, the job related to the machine, the machine drawings and specifications, and the sales order and hands it off to the Floor Manager for Production. After the Floor manager has all this information, he decides which machines to cut and in what order to cut them in based on shipping dates. He then takes the machine programs, jobs, and drawing and pass them out to the proper departments which will work on cutting, assembling, painting, and testing each one of our machines before they are sealed and shipped off to any of our customers throughout the world. Information flow is essential in any business in maintain functionality as well as productivity. Without some sort of order, without some sort of standard operating procedures our company could not be one of the leading manufacturer of agricultural machinery in the world.

Free Essays on Alcoholism

One evening a group of friends and I were sitting at my house watching movies. As we were all sitting there we suddenly heard someone banging on my door, I couldn’t think of who it would be at that time of night. As I looked through the window, I saw my Uncle Jim standing there. All I was able to notice when I opened the door was his bloodshot eyes and the strong odor of alcohol coming from his breath. As he was walking up my hallway stairs, I can only remember him stumbling, and stating â€Å"There’s no way I can go home†. Even though having been around alcoholics in my life, I have never truly understood what makes some people so attached to the alcohol. Many people today feel that alcoholism is an addiction which is a settled habit, but I feel that alcoholism is considered a progressive disease where a person consumes excessive amounts of alcohol and is unable to control his/her need to drink (Encyclopedia of Psychology 111-115). This is because alcoholism has been classified as a disease by the American Medical Society as well as by the National Council of Alcoholism because of the four factors such as it has symptoms and signs, it is diagnosable, it is progressive, and it can be treated (Facts about alcohol and Alcoholism 8). Drinking alcohol is an individual choice. People may drink for many different reasons. A few examples of why people drink are to be more sociable, be relaxed, and to feel bigger or stronger (Kinney & Leaton 8). It does, however become a problem when a person has an overwhelming compulsion to drink. This is when they are considered to be an alcoholic. Alcoholism has many early warning signs and symptoms, any one of which a person can signal a developing problem. Some of the symptoms consist of gulping the alcohol beverage in large amounts, or hiding the amount of alcohol they consumed from others. For example, keeping bottles stashed in the trunk of a car, or the m... Free Essays on Alcoholism Free Essays on Alcoholism Introduction When examining societies ‘normal’ families today we see them striving for values of love, loyalty and trust. Television shows and movies most likely illustrate teenagers drinking at ‘fun’ parties, or the parents at a social occasion, never getting trapped in the evils of drinking. Many people do not realize in the real world that these social ways of interacting can lead to much more serious and deeper issues in ones life. The causes and development of drinking are unbelievable, and it’s amazing that we don’t hear enough of this information in the media. This is the subject which I wish to discuss in this paper, and to define the true meaning of alcoholism: It refers to the drinking of alcoholic beverages to such a degree that it seriously and repeatedly interferes with major aspects of an individual’s life-such as work, school, family relations, or personal safety and health. Alcoholism is considered to be a disease, meaning that if it follows a characteristic course with known physical, psychological, and social symptoms. The alcoholic continues to consume alcohol despite the destructive consequences. (Acoholism-Encyclopedia; 427) How families deal with the stresses of a loved ones drinking habits are crucial to the way the child will learn to deal with drinking in the future. The effects of alcohol on the human body vary from person to person, as do the symptoms, and causes. The social effects of alcoholism will be reflected in this essay. Alcoholism in the family can cause many problems for each individual with in it. Causes and Symptoms â€Å"It has been said that there are as many causes for alcoholism as there are alcoholics.† (Ogilvie; 3) some of these are as follows alcoholics usually resort to drinking to ease anxiety; to heighten their excitement, to get them in a more upbeat mood; to enhance the feeling of power- so they feel they are in charge and they also may get aggressiv... Free Essays on Alcoholism Alcoholism refers to the abuse of alcohol by individuals who are unable to control their binge drinking behavior over a prolonged period of time. Alcoholics are not simply people who consume alcohol; instead, their entire lives revolve around alcohol. While many people usually dismiss the effects of heavy drinking to a hangover that will not last beyond the day, the effects of alcoholism are infinitely more enduring and devastating not only for the alcoholics, but also for their families and friends. Excessive consumption of alcohol can exert a severe impact on the brain, both on the short-term and long-term basis. The reason why alcoholics exhibit aggressive behavior can be attributed to the effects of alcohol on various parts of the brain. First, alcohol can affect the gamma-aminobutyoric acid receptor (GABA-A) complex in the brain that inhibits aggressive behavior by creating anxiety over socially inappropriate behavior. Second, the effect of alcohol on the dopaminergic system that controls the psychomotor stimulation can lead to an increase in the intensity and level of aggression. The lower blood sugar in the brain can also contribute to a heightened level of aggression (Graham, Wells, & West, 1997, p. 626). Consequently, alcoholics tend to overreact to unpleasant situations by using aggression. Furthermore, with excessive alcohol consumption, alcoholics lose their capacity to exercise self-control over their emotions and feelings. Very often, alcohol consumption becomes a means for them to unleash pent-up negative feelings. For other alcoholics, alcohol is a way for them to bury their negative feelings of anger, guilt and depression. Therefore, their general state of mind is moody and hostile, leading to increased chances of aggressive behavior at the slightest provocation (Graham, Wells, & West, 1997, p. 627). Alcohol also has debilitating effects on the individuals’ ability to function effectively in a cognitive way. Al... Free Essays on Alcoholism Alcoholism is a terrible disease, which has the possibility of affecting one’s health, family or work. College students often times turn to alcohol to solve their problems as a release from mounting school pressure. But does drinking while attending school really affect a student’s behavior pattern and performance in school? Studies indicate that as many as 30% of youth engage in frequent drinking behaviors (Sullivan & Risler, 2002). Believe it or not, this happens to be a more than a quarter of our student population! Granted learning can be tedious at times, but this probably could be the last stop of acquiring raw knowledge before heading out into the real world. I have assembled a set of factors that might explain student’s alcohol abuse and how it affects their behaviors respectively. Too bad it could be as easy as telling them to just stop drinking. A substantial amount of empirical research is available demonstrating a connection between alcohol consumption and impaired academic performance (Perkins, 2002). How surprising is this though? This whole statement reminds me of a chain reaction down the line. Play with fire and you die by fire. Drinking all night causes a student to lose interest in school. Their grades start to falter and laziness turns into major procrastination. A survey was taken from a pool of about 40,000 students stating that 22% had performed poorly on a test or project and 28% had missed a class due to alcohol use (Presley et al., 1996). One of the effects of student drinking is the increase of risky sexual behavior. In a study relating alcohol and sexual behavior (Desiderato & Crawford), 59% of students surveyed responded saying alcohol consumption usually preceded a sexually activity. This is surprisingly scary since sexual situations can lead to altered lives down the road. People tend to become less careless which leads to either unwanted pregnancy or even worse by contracting... Free Essays on Alcoholism The Psychosocial Effects of Alcoholism Alcoholism refers to the abuse of alcohol by individuals who are unable to control their binge drinking behavior over a prolonged period of time. Alcoholics are not simply people who consume alcohol; instead, their entire lives revolve around alcohol. While many people usually dismiss the effects of heavy drinking to a hangover that will not last beyond the day, the effects of alcoholism are infinitely more enduring and devastating not only for the alcoholics, but also for their families and friends. Excessive consumption of alcohol can exert a severe impact on the brain, both on the short-term and long-term basis. The reason why alcoholics exhibit aggressive behavior can be attributed to the effects of alcohol on various parts of the brain. First, alcohol can affect the gamma-aminobutyoric acid receptor (GABA-A) complex in the brain that inhibits aggressive behavior by creating anxiety over socially inappropriate behavior. Second, the effect of alcohol on the dopaminergic system that controls the psychomotor stimulation can lead to an increase in the intensity and level of aggression. The lower blood sugar in the brain can also contribute to a heightened level of aggression (Graham, Wells, & West, 1997, p. 626). Consequently, alcoholics tend to overreact to unpleasant situations by using aggression. Furthermore, with excessive alcohol consumption, alcoholics lose their capacity to exercise self-control over their emotions and feelings. Very often, alcohol consumption becomes a means for them to unleash pent-up negative feelings. For other alcoholics, alcohol is a way for them to bury their negative feelings of anger, guilt and depression. Therefore, their general state of mind is moody and hostile, leading to increased chances of aggressive behavior at the slightest provocation (Graham, Wells, & West, 1997, p. 627). Alcohol also has debilitating effects on the individuals’ ability to... Free Essays on Alcoholism One evening a group of friends and I were sitting at my house watching movies. As we were all sitting there we suddenly heard someone banging on my door, I couldn’t think of who it would be at that time of night. As I looked through the window, I saw my Uncle Jim standing there. All I was able to notice when I opened the door was his bloodshot eyes and the strong odor of alcohol coming from his breath. As he was walking up my hallway stairs, I can only remember him stumbling, and stating â€Å"There’s no way I can go home†. Even though having been around alcoholics in my life, I have never truly understood what makes some people so attached to the alcohol. Many people today feel that alcoholism is an addiction which is a settled habit, but I feel that alcoholism is considered a progressive disease where a person consumes excessive amounts of alcohol and is unable to control his/her need to drink (Encyclopedia of Psychology 111-115). This is because alcoholism has been classified as a disease by the American Medical Society as well as by the National Council of Alcoholism because of the four factors such as it has symptoms and signs, it is diagnosable, it is progressive, and it can be treated (Facts about alcohol and Alcoholism 8). Drinking alcohol is an individual choice. People may drink for many different reasons. A few examples of why people drink are to be more sociable, be relaxed, and to feel bigger or stronger (Kinney & Leaton 8). It does, however become a problem when a person has an overwhelming compulsion to drink. This is when they are considered to be an alcoholic. Alcoholism has many early warning signs and symptoms, any one of which a person can signal a developing problem. Some of the symptoms consist of gulping the alcohol beverage in large amounts, or hiding the amount of alcohol they consumed from others. For example, keeping bottles stashed in the trunk of a car, or the m... Free Essays on Alcoholism Alcoholism refers to the abuse of alcohol by individuals who are unable to control their binge drinking behavior over a prolonged period of time. Alcoholics are not simply people who consume alcohol; instead, their entire lives revolve around alcohol. While many people usually dismiss the effects of heavy drinking to a hangover that will not last beyond the day, the effects of alcoholism are infinitely more enduring and devastating not only for the alcoholics, but also for their families and friends. Excessive consumption of alcohol can exert a severe impact on the brain, both on the short-term and long-term basis. The reason why alcoholics exhibit aggressive behavior can be attributed to the effects of alcohol on various parts of the brain. First, alcohol can affect the gamma-aminobutyoric acid receptor (GABA-A) complex in the brain that inhibits aggressive behavior by creating anxiety over socially inappropriate behavior. Second, the effect of alcohol on the dopaminergic system that controls the psychomotor stimulation can lead to an increase in the intensity and level of aggression. The lower blood sugar in the brain can also contribute to a heightened level of aggression (Graham, Wells, & West, 1997, p. 626). Consequently, alcoholics tend to overreact to unpleasant situations by using aggression. Furthermore, with excessive alcohol consumption, alcoholics lose their capacity to exercise self-control over their emotions and feelings. Very often, alcohol consumption becomes a means for them to unleash pent-up negative feelings. For other alcoholics, alcohol is a way for them to bury their negative feelings of anger, guilt and depression. Therefore, their general state of mind is moody and hostile, leading to increased chances of aggressive behavior at the slightest provocation (Graham, Wells, & West, 1997, p. 627). Alcohol also has debilitating effects on the individuals’ ability to function effectively in a cognitive way. Al... Free Essays on Alcoholism Alternative names alcohol dependence; habitual alchohol use Definition A illness marked by uncontrolled consumption of alcoholic beverages that interferes with physical or mental health, and social, family, or occupational responsibilities. Causes, incidence, and risk factors Alcoholism is a type of drug dependence. There is both physical and psychological dependence with this addiction. Physical dependence reveals itself in withdrawal symptoms when alcohol intake is interrupted, tolerance to the effects of alcohol, and evidence of alcohol-associated illnesses. Alcohol affects the central nervous system as a depressant resulting in a decrease of activity, , , and inhibitions. Even a low level of alcohol within the body slows reactions. Concentration and judgment become impaired. In excessive amounts, intoxication, or poisoning results. Alcohol also affects other body systems. Irritation of the gastrointestinal tract can occur with of the lining of the stomach causing . are not absorbed properly, which can lead to nutritional deficiencies with the long-term use of alcohol. , called , may also develop. The system may be affected by . Sexual dysfunction can also occur, causing erectile dysfunction in men and in women. during can cause problems in the developing fetus known as fetal alcohol syndrome. The development of dependence upon alcohol may occur over 5 to 25 years, following a relatively consistent pattern of progression. At first, a tolerance of alcohol develops. This results in a person being able to consume a greater quantity of alcohol before its adverse effects are noticed. Memory lapses relating to drinking episodes may follow tolerance. Then a lack of control over drinking occurs, and the affected person can no longer discontinue drinking whenever desired. The most severe drinking behavior includes prolonged binges of drinking with associated mental or physical complications. Some peo... Free Essays on Alcoholism Alcoholism Alcohol is a liquid distilled product of fermented fruits, grains and vegetables used as a solvent, antiseptic and sedative for potential abuse (Webster’s New Word Dictionary). Many people drink for the effects of sensory alteration and anxiety reduction. Drinking too much of this substance can cause staggering, loss of coordination, slurred speech, nerve damage, liver damage, and dilated pupils. An alcoholic has problems admitting that alcoholism is a disease, and that they are addicted to this substance. Alcoholism has been called the most serious drug problem in terms of the number of victims and costs to society. Alcohol is a depressant that will release feelings of guilt, anxiety and remorse if taken in heavy quantities. It will impair your motor skills by slowing your alertness and awareness, which leads to many accidents. Recent studies have shown that 50 to 80% of all alcoholics have close relatives that are alcoholics as well. In 1990 scientists found what they believe to be responsible for the inheritance of alcoholism in family lines. The defective gene is located on chromosome 11 and is called Dopamine. Dopamine is a receptor located in the brain. People with fewer Dopamine receptors are very likely to develop alcoholism. This helps to develop the theory that alcoholism is not a disease of choice, as believed by many. An estimated ten million Americans suffer from this horrible disease. There are many signs that may lead to the development of an alcoholic. Most alcoholics feel the need to take a drink just as a smoker has a craving for cigarettes. In the later stages, people have been known to suffer from frequent blackouts, which have led to many accidents. It is estimated that there are three million alcoholics between the ages of fourteen and seventeen today. Drinking does not only affect the alcoholic, it affects the family and society as well. An estimated forty billion dollars is spe...